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Heregulin beta 1 antibody

Reactivity: Human ELISA, WB, Neut Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN5690593
  • Target See all Heregulin beta 1 products
    Heregulin beta 1
    Reactivity
    Human
    Host
    • 1
    Rabbit
    Clonality
    • 1
    Polyclonal
    Conjugate
    • 1
    This Heregulin beta 1 antibody is un-conjugated
    Application
    ELISA, Western Blotting (WB), Neutralization (Neut)
    Immunogen
    Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin-B1. Human Heregulin-B1 specific antibody was purified by affinity chromatography employing an immobilized Human Heregulin-B1 matrix.
  • Application Notes
    Neutralization:
    To yield one-half maximal inhibition [ND50] of the biological activity of hHeregulin-B1 (1.0 ng/mL), a concentration of 0.1-1.1 μ,g/mL of this antibody is required.

    ELISA:

    In a sandwich ELISA To detect hHeregulin-B1 (using 100 uL/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction withour biotinylated Anti-Human hHeregulin-B1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hHeregulin-B1.

    Western Blot:
    To detect hHeregulin-B1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hHeregulin-B1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Storage
    -20 °C
    Storage Comment
    The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
  • Target
    Heregulin beta 1
    Alternative Name
    Heregulin-beta1 (Heregulin beta 1 Products)
    Gene ID
    3084
    UniProt
    Q02297
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