ApoE3 antibody (Biotin)

Catalog No. ABIN5690605

The Rabbit Polyclonal anti-ApoE3 antibody is suitable to detect ApoE3 in samples from Human. It has been validated for ELISA and WB.

Quick Overview for ApoE3 antibody (Biotin) (ABIN5690605)

Target: ApoE3 (Apolipoprotein E3 (ApoE3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ApoE3 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-ApoE3 Antibody (Biotin)

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hApoE3. Human ApoE3 specific antibody was purified by affinity chromatography and then biotinylated. *Cross Reactivity: human ApoE2, human ApoE4 in both western blot and sandwich ELISA

Application Details

Application Notes: ELISA:
Direct:
To detect hApoE3 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Sandwich
To detect hApoE3 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human ApoE3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hApoE3.

Western Blot:
To detect hApoE3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hApoE3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: ApoE3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for ApoE3

Target: ApoE3 (Apolipoprotein E3 (ApoE3))

Alternative Name: ApoE3

Gene ID: 348

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism