DLL4 antibody (Biotin)

Catalog No. ABIN5690608

The Rabbit Polyclonal anti-DLL4 antibody has been validated for WB and ELISA. It is suitable to detect DLL4 in samples from Human.

Quick Overview for DLL4 antibody (Biotin) (ABIN5690608)

Target: DLL4 (delta-Like 4 (DLL4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLL4 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-DLL4 Antibody (Biotin)

Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human sDLL-4. Human sDLL-4 specific antibody was purified by affinity chromatography and then biotinylated.

Application Details

Application Notes: ELISA:
Direct:
To detect hsDLL-4 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsDLL-4.

Sandwich
To detect hsDLL-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci's Polyclonal Anti-Human sDLL-4 (38-269) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsDLL-4.

Western Blot:

To detect hsDLL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsDLL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Target Details for DLL4

Target: DLL4 (delta-Like 4 (DLL4))

Alternative Name: sDLL-4

Gene ID: 54567

UniProt: Q9NR61

Pathways: Notch Signaling