APAF1 antibody (AA 1-97)

Catalog No. ABIN5690623

The Rat Monoclonal anti-APAF1 antibody is suitable to detect APAF1 in samples from Human and Mouse. It has been validated for WB, ELISA, IP and ICC.

Quick Overview for APAF1 antibody (AA 1-97) (ABIN5690623)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 18H2

Product Details anti-APAF1 Antibody

Binding Specificity: AA 1-97

Specificity: Recognizes the CARD domain of human and mouse Apaf-1. Does not cross-react with rat Apaf-1.

Purity: > 95% (SDS-PAGE)

Immunogen: Recombinant mouse Apaf-1 (aa 1-97) containing the N-terminal CARD domain.

Isotype: IgG2a kappa

Application Details

Application Notes: Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid. In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for at least 1 year after receipt when stored at -20°C.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf-1

Background: Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Gene ID: 11783

UniProt: O88879

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity