BAFF antibody (AA 83-285)

Catalog No. ABIN5690646

This Rat Monoclonal antibody specifically detects BAFF in WB, IHC and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for BAFF antibody (AA 83-285) (ABIN5690646)

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This BAFF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: Buffy-2

Product Details anti-BAFF Antibody

Binding Specificity: AA 83-285

Purification: Anti BAFF (monoclonal Buffy-2) antibody was purified to ≥,95% in SDS-PAGE.

Immunogen: BAFF (monoclonal Buffy-2) antibody was raised against recombinant human soluble BAFF (aa. 83-285).

Isotype: IgG2a

Application Details

Application Notes: BAFF antibody can be used to recognize membrane-bound and soluble human and mouse (weak) BAFF by Flow Cytometry, Immunohistochemistry (frozen sections, paraffin sections) and Western Blot. Method: HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of full length hBAFF (thick line). Cells were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % fetal calf serum, 0.02 % azide) containing 1 μ,g/mL of MAb to BAFF (Buffy-2) (FITC). After washing, cells were analyzed by flow cytometry. Method: Tissues are in paraffin sections. Sections are de-waxed and rehydrated, then cooked. Sections are stained in wet boxes, rehydrated and finally prepared as coverslip slides using Eukitt solution. Please inquire for complete protocol..

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: BAFF antibody can be stored at 4°C, stable for one year.

Target Details for BAFF

Target: BAFF (TNFSF13B) (Tumor Necrosis Factor (Ligand) Superfamily, Member 13b (TNFSF13B))

Alternative Name: BAFF

Gene ID: 10673

UniProt: Q9Y275

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response