AKR1C2 antibody (AA 1-123)

Catalog No. ABIN5692785

This Rabbit Polyclonal antibody specifically detects AKR1C2 in WB, IF, ICC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for AKR1C2 antibody (AA 1-123) (ABIN5692785)

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKR1C2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-AKR1C2 Antibody

Binding Specificity: AA 1-123

Purpose: Anti-AKR1C1/C2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AKR1C1/C2 Antibody Picoband® (ABIN5692785). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human AKR1C1/C2 recombinant protein (Position: M1-K123).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Ciaccio PJ, Tew KD (Jun 1994). "cDNA and deduced amino acid sequences of a human colon dihydrodiol dehydrogenase". Biochimica et Biophysica Acta 1186 (1-2): 129-32. 2. O'connor T, Ireland LS, Harrison DJ, Hayes JD (Oct 1999). "Major differences exist in the function and tissue-specific expression of human aflatoxin B1 aldehyde reductase and the principal human aldo-keto reductase AKR1 family members". The Biochemical Journal 343 (2): 487-504.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AKR1C2

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Alternative Name: AKR1C2

Background:

Synonyms: Aldo-keto reductase family 1 member C2,1.-.-.-,3-alpha-HSD3,Chlordecone reductase homolog HAKRD,Dihydrodiol dehydrogenase 2,DD-2,DD2,Dihydrodiol dehydrogenase/bile acid-binding protein,DD/BABP,Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase,1.3.1.20 ,Type III 3-alpha-hydroxysteroid dehydrogenase,1.1.1.357 ,AKR1C2,DDH2,

Tissue Specificity: Expressed in fetal testes. Expressed in fetal and adult adrenal glands. .

Background: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. And this gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

Molecular Weight: 37 kDa

Gene ID: 1645, 1646

UniProt: P52895

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process