AKR1C2 antibody

Catalog No. ABIN6939232

The Mouse Monoclonal anti-AKR1C2 antibody is suitable to detect AKR1C2 in samples from Human. It has been validated for WB and IHC.

Quick Overview for AKR1C2 antibody (ABIN6939232)

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKR1C2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPTC-AKR1C2-1

Product Details anti-AKR1C2 Antibody

Purpose: Aldo-keto Reductase Family 1 Member C2 / DD2 Antibody

Specificity: DDH2 / AKR1C2 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length AKR1C2 protein

Isotype: IgG2a, kappa

Application Details

Application Notes:

Positive Control: HeLa, K-562, A431, HepG2, A549 cells. Human liver or stomach tissue.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for AKR1C2

Target: AKR1C2 (Aldo-keto Reductase Family 1, Member C2 (AKR1C2))

Alternative Name: AKR1C2

Background:

AKR1C2, 3-alpha-HSD3, AKR1C-pseudo protein, BABP, Dihydrodiol dehydrogenase 2, DD-2, MCDR2, HAKRD, DD, DDH2, HBAB, Pseudo-chlordecone reductase, SRXY8

A431, A549 cells. Human liver or stomach tissue., HeLa, Hep G2, K-562

Molecular Weight: 37kDa

Gene ID: 1646, 567256

UniProt: P52895

Pathways: Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process