Bestrophin 1 antibody

Catalog No. ABIN5692801

The Rabbit Polyclonal anti-Bestrophin 1 antibody has been validated for WB, IHC, FACS and ICC. It is suitable to detect Bestrophin 1 in samples from Human.

Quick Overview for Bestrophin 1 antibody (ABIN5692801)

Target: Bestrophin 1 (BEST1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bestrophin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Bestrophin 1 Antibody

Purpose: Anti-Bestrophin/BEST1 Antibody Picoband®

Sequence: RFIYRLALTE EQQLMFEKLT LYCD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Bestrophin/BEST1 Antibody Picoband® (ABIN5692801). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Bestrophin, which shares 62.5% amino acid (aa) sequence identity with mouse Bestrophin.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. "Entrez Gene: BEST1 bestrophin 1". 2. Johnson AA, Guziewicz KE, Lee CJ, Kalathur RC, Pulido JS, Marmorstein LY, Marmorstein AD (January 2017). "Bestrophin 1 and retinal disease". review. Progress in Retinal and Eye Research. 58: 45-69.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Bestrophin 1

Target: Bestrophin 1 (BEST1)

Alternative Name: BEST1

Background:

Synonyms: Bestrophin-1, TU15B, Vitelliform macular dystrophy protein 2, BEST1, VMD2

Tissue Specificity: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.

Background: Bestrophin-1 (Best1) is a protein that, in humans, is encoded by the BEST1 gene. This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.

Molecular Weight: 68 kDa

Gene ID: 7439

UniProt: O76090