Betacellulin antibody (AA 32-177)

Catalog No. ABIN5692803

This Rabbit Polyclonal antibody specifically detects Betacellulin in WB, IHC and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Betacellulin antibody (AA 32-177) (ABIN5692803)

Target: Betacellulin (BTC)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Betacellulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Betacellulin Antibody

Binding Specificity: AA 32-177

Purpose: Anti-Betacellulin/Btc Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Betacellulin/Btc Antibody Picoband® (ABIN5692803). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived rat Betacellulin recombinant protein (Position: D32-A177).

Isotype: IgG

Application Details

Application Notes: Western blot,0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section),0.5-1 μg/mL
ELISA,0.1-0.5 μg/mL
1. Elbein SC, Wang X, Karim MA, et al. (2006). "Analysis of coding variants in the betacellulin gene in type 2 diabetes and insulin secretion in African American subjects.". BMC Med. Genet. 7: 62. 2. Kim HS, Shin HS, Kwak HJ, Cho CH, Lee CO, Koh GY (February 2003). "Betacellulin induces angiogenesis through activation of mitogen-activated protein kinase and phosphatidylinositol 3'-kinase in endothelial cell". FASEB J. 17 (2): 318-20.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Betacellulin

Target: Betacellulin (BTC)

Alternative Name: Btc

Background:

Synonyms: Betacellulin

Tissue Specificity: Highly expressed in testis.

Background: Betacellulin is a protein that in humans is encoded by the BTC gene located on chromosome 4 at locus 4q13-q21. This gene encodes a member of the epidermal growth factor (EGF) family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the secreted growth factor. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. This protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability. Mutations in this gene may be associated with type 2 diabetes in human patients.

Molecular Weight: 26 kDa

Gene ID: 64022

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway