VCAM1 antibody (AA 25-270)

Catalog No. ABIN5692810

The Rabbit Polyclonal anti-VCAM1 antibody has been validated for WB and ELISA. It is suitable to detect VCAM1 in samples from Human. There are 7+ publications available.

Quick Overview for VCAM1 antibody (AA 25-270) (ABIN5692810)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-VCAM1 Antibody

Binding Specificity: AA 25-270

Purpose: Anti-VCAM1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-VCAM1 Antibody Picoband® (ABIN5692810). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human VCAM1 recombinant protein (Position: F25-L270).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA (Cap), 1-5 μg/mL
1. Cybulsky, M., Fries, J. W., Williams, A. J., Sultan, P., Eddy, R. L., Byers, M. G., Shows, T. B., Gimbrone, M. A., Jr., Collins, T. The human VCAM1 gene is assigned to chromosome 1p31-p32. (Abstract)Cytogenet. Cell Genet. 58: 1852, 1991. 2. Kumar, A. G., Dai, X. Y., Kozak, C. A., Mims, M. P., Gotto, A. M., Ballantyne, C. M. Murine VCAM-1: molecular cloning, mapping, and analysis of a truncated form. J. Immun. 153: 4088-4098, 1994. 3. Taylor, J. G., VI, Tang, D. C., Savage, S. A., Leitman, S. F., Heller, S. I., Serjeant, G. R., Rodgers, G. P., Chanock, S. J. Variants in the VCAM1 gene and risk for symptomatic stroke in sickle cell disease. Blood 100: 4303-4309, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-VCAM1 antibody (ABIN5692810)

Ma, Pan, Ren, Guo, Guo, Wei, Zheng, Chen: "15-oxoeicosatetraenoic acid mediates monocyte adhesion to endothelial cell." in: Lipids in health and disease, Vol. 16, Issue 1, pp. 137, (2018) (PubMed).

Wang, Qing, Liu, Liu, Qiao, Cui, He, Zhao, Liu, Yan, Wang, Liang, Guo, Shen, Hou, Chen: "Mesenchymal stromal cells ameliorate oxidative stress-induced islet endothelium apoptosis and functional impairment via Wnt4-β-catenin signaling." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 188, (2018) (PubMed).

Hoffman, Adeli: "LDL Receptor Gene-Ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia with Dominant Inheritance and Diet-Induced Coronary Atherosclerosis." in: EBioMedicine, Vol. 28, pp. 17-18, (2018) (PubMed).

Tian, Tao, Zhao, Tai, Liu, Liu: "Isolation and morphological characterization of ovine amniotic fluid mesenchymal stem cells." in: Experimental animals, Vol. 65, Issue 2, pp. 125-34, (2017) (PubMed).

Ma, Pan, Chen, Guo, Zhao, Zheng, Chen: "Trimethylamine N-oxide in atherogenesis: impairing endothelial self-repair capacity and enhancing monocyte adhesion." in: Bioscience reports, Vol. 37, Issue 2, (2017) (PubMed).

Chen, Zhang, Chen, Liu, Wang, Zhou, Qiu, Zhang, Teng, Mao, Liu, Wei, Li: "Retinoic Acid promotes interleukin-4 plasmid-dimethylsulfoxide topical transdermal delivery for treatment of psoriasis." in: Annals of dermatology, Vol. 27, Issue 2, pp. 121-7, (2015) (PubMed).

Yu, Ge, Summers, Li, Liu, Ma, Kaminski, Zhuang: "TSP-1 secreted by bone marrow stromal cells contributes to retinal ganglion cell neurite outgrowth and survival." in: PLoS ONE, Vol. 3, Issue 6, pp. e2470, (2008) (PubMed).

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: VCAM1

Background:

Synonyms: Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106, VCAM1

Tissue Specificity: Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.

Background: CD106 (cluster of differentiation 106) also known as vascular cell adhesion molecule 1 (VCAM-1), is a protein that in humans is encoded by the VCAM1 gene. In inflammatory conditions and in cardiac allografts undergoing rejection, VCAM1 is upregulated in endothelium of postcapillary venules. Arterial expression of VCAM1 is also found in experimental models of atherosclerosis in the rabbit. This gene is mapped to chromosome 1 by Southern analysis of somatic cell hybrids. VCAM-1 functions as a cell adhesion molecule. The VCAM-1 protein mediates the adhesion of lymphocytes, monocytes, eosinophils, andbasophils to vascularendothelium. It also functions in leukocyte-endothelial cell signal transduction, and it may play a role in the development ofatherosclerosis and rheumatoid arthritis. CAM741 works similar to cotransin in that it represses the biosynthesis of VCAM1 cells by blocking the process of cotranslational translocation, which is dependent on the signal peptide ofVCAM1. Among the lung metastasis signature genes identified, several, includingVCAM1, were functionally validated.

Molecular Weight: 95 kDa

Gene ID: 7412

UniProt: P19320

Pathways: Carbohydrate Homeostasis