Topoisomerase I antibody (AA 526-765)

Catalog No. ABIN5692844

This anti-Topoisomerase I antibody is a Rabbit Polyclonal antibody detecting Topoisomerase I in WB, ELISA, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for Topoisomerase I antibody (AA 526-765) (ABIN5692844)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Topoisomerase I Antibody

Binding Specificity: AA 526-765

Purpose: Anti-Topoisomerase I/TOP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Topoisomerase I/TOP1 Antibody Picoband® (ABIN5692844). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Topoisomerase I recombinant protein (Position: E526-F765).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: TOP1 topoisomerase (DNA) I". 2. Ahuja, H. G., Felix, C. A., Aplan, P. D. The t(11,20)(p15,q11) chromosomal translocation associated with therapy-related myelodysplastic syndrome results in an NUP98-TOP1fusion. Blood 94: 3258-3261, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: TOP1

Background:

Synonyms: DNA topoisomerase 1, DNA topoisomerase I, TOP1,

Tissue Specificity: Endothelial cells.

Background: DNA topoisomerase 1 is an enzyme that in humans is encoded by the TOP1 gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Molecular Weight: 100 kDa

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance