Phospholipase C gamma 2 antibody (AA 1201-1258)

Catalog No. ABIN5692854

This Rabbit Polyclonal antibody specifically detects Phospholipase C gamma 2 in WB, IHC, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for Phospholipase C gamma 2 antibody (AA 1201-1258) (ABIN5692854)

Target: Phospholipase C gamma 2 (PLCG2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-Phospholipase C gamma 2 Antibody

Binding Specificity: AA 1201-1258

Purpose: Anti-PLCG 2/PLCG2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PLCG 2/PLCG2 Antibody Picoband® (ABIN5692854). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human PLCG 2 recombinant protein (Position: R1201-V1258).

Isotype: IgG

Application Details

Application Notes: Western blot,0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section),2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA,0.1-0.5 μg/mL
1. "Entrez Gene: PLCG2 phospholipase C, gamma 2 (phosphatidylinositol-specific)". 2. Hernandez D, Egan SE, Yulug IG, Fisher EM (February 1995). "Mapping the gene that encodes phosphatidylinositol-specific phospholipase C-gamma 2 in the human and the mouse". Genomics. 23 (2): 504-7.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Alternative Name: PLCG2

Background:

Synonyms: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV, PLC-IV, Phospholipase C-gamma-2, PLC-gamma-2, PLCG2

Background: 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 is an enzyme that in humans is encoded by the PLCG2 gene. The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.

Molecular Weight: 148 kDa

Gene ID: 5336

UniProt: P16885

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling