NADPH Oxidase 4 antibody

Catalog No. ABIN5692900

The Rabbit Monoclonal anti-NADPH Oxidase 4 antibody has been validated for WB, IHC, IF, IP and ICC. It is suitable to detect NADPH Oxidase 4 in samples from Human, Rat and Mouse. There are 2+ publications available.

Quick Overview for NADPH Oxidase 4 antibody (ABIN5692900)

Target: NADPH Oxidase 4 (NOX4)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NADPH Oxidase 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: E-14

Product Details anti-NADPH Oxidase 4 Antibody

Purpose: Anti-NOX4 Rabbit Monoclonal Antibody

Characteristics: Anti-NOX4 Rabbit Monoclonal Antibody (ABIN5692900). Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human NOX4

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

References for anti-NADPH Oxidase 4 antibody (ABIN5692900)

Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." in: Antioxidants & redox signaling, (2018) (PubMed).

Zhao, Li, Liu, Zhang, Zhang, Zeng, Wu, Gao, Lan, Wang: "Polydatin protects against carbon tetrachloride-induced liver fibrosis in mice." in: Archives of biochemistry and biophysics, Vol. 629, pp. 1-7, (2017) (PubMed).

Target Details for NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Alternative Name: NOX4

Background:

Synonyms: NADPH oxidase 4,1.6.3.-,Kidney oxidase-1,KOX-1,Kidney superoxide-producing NADPH oxidase,Renal NAD (P)H-oxidase,NOX4,RENOX,

Tissue Specificity: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells. .

Molecular Weight: 52 kDa

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration