PON1 antibody (AA 30-274)

Catalog No. ABIN5692913

This anti-PON1 antibody is a Mouse Monoclonal antibody detecting PON1 in WB, IHC and ELISA. Suitable for Mouse and Rat.

Quick Overview for PON1 antibody (AA 30-274) (ABIN5692913)

Target: PON1 (Paraoxonase 1 (PON1))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PON1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 9D3

Product Details anti-PON1 Antibody

Binding Specificity: AA 30-274

Purpose: Anti-PON1 Antibody Picoband® (monoclonal, 9D3)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PON1 Antibody Picoband® (monoclonal, 9D3) (ABIN5692913). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA (Cap), 1-5 μg/mL1. Costa LG, Cole TB, Vitalone A, Furlong CE (2005). "Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity". CLINICA CHEMICA ACTA 352 (1-2): 37-47. 2. Khateeb J, Gantman A, Kreitenberg AJ, Aviram M, Fuhrman B (January 2010). "Paraoxonase 1 (PON1) expression in hepatocytes is upregulated by pomegranate polyphenols: a role for PPAR-gamma pathway".Atherosclerosis 208 (1): 119-25. 3. Primo-Parmo SL, Sorenson RC, Teiber J, La Du BN (May 1996). "The human serum paraoxonase/arylesterase gene (PON1) is one member of a multigene family". Genomics 33 (3): 498-507.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PON1

Target: PON1 (Paraoxonase 1 (PON1))

Alternative Name: Pon1

Background:

Synonyms: Serum paraoxonase/arylesterase 1, PON 1

Tissue Specificity: Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Background: Isocitrate dehydrogenase [NADP], mitochondrialis anenzymethat in humans is encoded by theIDH2gene. Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD (+) as the electron acceptor and the other NADP (+). Five isocitrate dehydrogenases have been reported: three NAD (+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP (+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP (+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP (+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants.

Molecular Weight: 40 kDa

Gene ID: 18979

UniProt: P52430