SYT1 antibody

Catalog No. ABIN5692948

The Rabbit Polyclonal anti-SYT1 antibody has been validated for WB, IHC, ICC and FACS. It is suitable to detect SYT1 in samples from Human, Rat and Mouse.

Quick Overview for SYT1 antibody (ABIN5692948)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SYT1 Antibody

Purpose: Anti-Synaptotagmin 1/SYT1 Antibody Picoband®

Sequence: RRPIAQWHTL QVEEEVDAML AVKK

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Synaptotagmin 1/SYT1 Antibody Picoband® (ABIN5692948). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Synaptotagmin 1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells1. Fernandez-Chacon, R., Konigstorfer, A., Gerber, S. H., Garcia, J., Matos, M. F., Stevens, C. F., Brose, N., Rizo, J., Rosenmund, C., Sudhof, T. C. Synaptotagmin I functions as a calcium regulator of release probability. Nature 410: 41-49, 2001. 2. Poskanzer, K. E., Marek, K. W., Sweeney, S. T., Davis, G. W. Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature 426: 559-563, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: SYT1

Background:

Synonyms: Synaptotagmin-1, Synaptotagmin I, SytI, p65, SYT1, SVP65, SYT

Tissue Specificity: Expressed in melanocytes (PubMed:23999003).

Background: Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.

Molecular Weight: 65 kDa

Gene ID: 6857

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport