CCT7 antibody (AA 30-307)

Catalog No. ABIN5692966

The Rabbit Polyclonal anti-CCT7 antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect CCT7 in samples from Human, Mouse and Rat.

Quick Overview for CCT7 antibody (AA 30-307) (ABIN5692966)

Target: CCT7 (Chaperonin Containing TCP1, Subunit 7 (Eta) (CCT7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-CCT7 Antibody

Binding Specificity: AA 30-307

Purpose: Anti-TCP1 eta/CCT7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TCP1 eta/CCT7 Antibody Picoband® (ABIN5692966). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human CCT7 recombinant protein (Position: Q30-D307).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: CCT7 chaperonin containing TCP1, subunit 7 (eta)". 2. Won KA, Schumacher RJ, Farr GW, Horwich AL, Reed SI (Dec 1998). "Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT". Molecular and Cellular Biology. 18 (12): 7584-9.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCT7

Target: CCT7 (Chaperonin Containing TCP1, Subunit 7 (Eta) (CCT7))

Alternative Name: CCT7

Background:

Synonyms: T-complex protein 1 subunit eta, TCP-1-eta, CCT-eta, HIV-1 Nef-interacting protein, T-complex protein 1 subunit eta, N-terminally processed, CCT7, CCTH, NIP7-1

Background: T-complex protein 1 subunit eta is a protein that in humans is encoded by the CCT7 gene. This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.

Molecular Weight: 59 kDa

Gene ID: 10574

UniProt: Q99832