alpha Fetoprotein antibody (AA 19-254)

Catalog No. ABIN5692976

Product Details anti-alpha Fetoprotein Antibody

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Binding Specificity: AA 19-254

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Alpha 1 Fetoprotein detection. Tested with WB, Direct ELISA in Mouse,Rat.

Immunogen: E. coli-derived mouse Alpha 1 Fetoprotein recombinant protein (Position: K19-L254).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-alpha Fetoprotein antibody (ABIN5692976)

Jeng, Jeng, Jeng, Sheen, Li, Lu, Chang: "Tropism of liver epithelial cells toward hepatocellular carcinoma in vitro and in vivo with altering gene expression of cancer stem cells." in: American journal of surgery, Vol. 215, Issue 4, pp. 735-743, (2017) (PubMed).

Winkler, Hempel, Brückner, Mallek, Weise, Liehr, Tautenhahn, Bartels, Christ: "Mouse white adipose tissue-derived mesenchymal stem cells gain pericentral and periportal hepatocyte features after differentiation in vitro, which are preserved in vivo after hepatic transplantation." in: Acta physiologica (Oxford, England), Vol. 215, Issue 2, pp. 89-104, (2016) (PubMed).

Yan, Zhu, Sun, Zhang, Li, Sun, Li, Qian, Zhu, Xu: "Extracellular regulated protein kinases 1/2 phosphorylation is required for hepatic differentiation of human umbilical cord-derived mesenchymal stem cells." in: Experimental biology and medicine (Maywood, N.J.), Vol. 240, Issue 4, pp. 534-45, (2015) (PubMed).

Shan, Wu, Li, Shen, Wang, Liu, Shen, Lei: "Continuous passages accelerate the reprogramming of mouse induced pluripotent stem cells." in: Cellular reprogramming, Vol. 16, Issue 1, pp. 77-83, (2014) (PubMed).

Wang, Xu, Zhou, Zhong, Wen, Yu, Chen, Shen, Chen, She, Jiang, Miao, Wei: "The viral oncoprotein HBx of Hepatitis B virus promotes the growth of hepatocellular carcinoma through cooperating with the cellular oncoprotein RMP." in: International journal of biological sciences, Vol. 10, Issue 10, pp. 1181-92, (2014) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Alternative Name: Afp (AFP Products)

Synonyms: FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody

Background:

Synonyms: Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Afp

Tissue Specificity: Plasma.

UniProt: P02772

Pathways: C21-Steroid Hormone Metabolic Process