Cadherin 5 antibody (AA 48-272)

Catalog No. ABIN5692986

Product Details anti-Cadherin 5 Antibody

Target: Cadherin 5 (CDH5)

Binding Specificity: AA 48-272

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cadherin 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for VE Cadherin detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human VE Cadherin recombinant protein (Position: D48-R272).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-Cadherin 5 antibody (ABIN5692986)

Dai, Chen, Lin, Wang, Guo, Zhang: "Exposure to Concentrated Ambient Fine Particulate Matter Induces Vascular Endothelial Dysfunction via miR-21." in: International journal of biological sciences, Vol. 13, Issue 7, pp. 868-877, (2018) (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, (2014) (PubMed).

Target Details for Cadherin 5

Target: Cadherin 5 (CDH5)

Alternative Name: CDH5 (CDH5 Products)

Synonyms: 7B4 antibody, AA408225 antibody, Cd144 antibody, VE-Cad antibody, VECD antibody, VEcad antibody, Vec antibody, wu:fc88a07 antibody, CD144 antibody, cadherin-5 antibody, cadherin 5 antibody, CDH5 antibody, Cdh5 antibody, cdh5 antibody

Background:

Synonyms: Cadherin-5, 7B4 antigen, Vascular endothelial cadherin, VE-cadherin, CD144, CDH5

Tissue Specificity: Endothelial tissues and brain.

Background: CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.

UniProt: P33151

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2