EPO antibody (AA 34-192)

Catalog No. ABIN5693048

The Rabbit Polyclonal anti-EPO antibody (ABIN5693048) specifically detects EPO in WB. The antibody is reactive with Mouse and Rat samples.

Quick Overview for EPO antibody (AA 34-192) (ABIN5693048)

Target: EPO (Erythropoietin (EPO))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EPO Antibody

Binding Specificity: AA 34-192

Purpose: Anti-EPO Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EPO Antibody (ABIN5693048). Tested in WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse EPO recombinant protein (Position: D34-R192).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: Epo

Target Type: Hormone

Background:

Synonyms: Erythropoietin, Epo

Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Background: This EPO gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies.

Molecular Weight: 28 kDa

Gene ID: 13856

UniProt: P07321

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity