PPID antibody (AA 306-370)

Catalog No. ABIN5693052

This Rabbit Polyclonal antibody specifically detects PPID in WB, IHC, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PPID antibody (AA 306-370) (ABIN5693052)

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-PPID Antibody

Binding Specificity: AA 306-370

Purpose: Anti-PPID Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PPID Antibody Picoband® (ABIN5693052). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human PPID recombinant protein (Position: N306-A370).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Kieffer, L. J., Thalhammer, T., Handschumacher, R. E.Isolation and characterization of a 40- kDa cyclophilin-related protein.J. Biol. Chem. 267: 5503-5507, 1992. 2. Ratajczak, T., Woollatt, E., Kumar, P., Ward, B. K., Minchin, R. F., Baker, E.Cyclophilin 40 (PPID) gene map position 4q31.3.Chromosome Res. 5: 151 only, 1997. 3. Yokoi, H., Shimizu, Y., Anazawa, H., Lefebvre, C. A., Korneluk, R. G., Ikeda, J.-E.The structure and complete nucleotide sequence of the human cyclophilin 40 (PPID) gene.Genomics 35: 448-455, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for PPID

Target: PPID (Peptidylprolyl Isomerase D (PPID))

Alternative Name: PPID

Background:

Synonyms: Peptidyl-prolyl cis-trans isomerase D, PPIase D

Tissue Specificity: Widely expressed.

Background: Cyclophilin D, Peptidylprolyl isomerase D, also known as PPID, is an enzyme which in humans is encoded by the PPID gene. The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. The Cyclophilin D (PPID) gene contains 10 exons and spans 14.2 kb of genomic DNA. By fluorescence in situ hybridization, the PPID gene is mapped to chromosome 4q31.3. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein has been shown to possess PPIase activity and, similar to other family members, can bind to the immunosuppressant ciclosporin.

Molecular Weight: 38 kDa

Gene ID: 5481

UniProt: Q08752

Pathways: Nuclear Hormone Receptor Binding