PRKAB2 antibody (N-Term)

Catalog No. ABIN5693058

This Mouse Monoclonal antibody specifically detects PRKAB2 in WB, IHC, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for PRKAB2 antibody (N-Term) (ABIN5693058)

Target: PRKAB2 (Protein Kinase, AMP-Activated, beta 2 Non-Catalytic Subunit (PRKAB2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKAB2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 6G1

Product Details anti-PRKAB2 Antibody

Binding Specificity: N-Term

Purpose: Anti-AMPK beta 2 PRKAB2 Antibody (monoclonal, 6G1)

Sequence: DKEFVSWQQD LEDSVKPTQQ ARPTVIRWSE GGKE

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by three amino acids,and from the related rat sequence by two amino acids.

Characteristics: Anti-AMPK beta 2 PRKAB2 Antibody.(monoclonal, 6G1). Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AMPK beta 2, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry, 0.5-1 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x106 cells

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKAB2

Target: PRKAB2 (Protein Kinase, AMP-Activated, beta 2 Non-Catalytic Subunit (PRKAB2))

Alternative Name: PRKAB2

Background:

Background: 5'-AMP-activated protein kinase subunit beta-2 is an enzyme that in humans is encoded by the PRKAB2 gene. The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. It is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles. Multiple alternatively spliced transcript variants have been found for this gene.

Gene Full Name: protein kinase AMP-activated non-catalytic subunit beta 2

Molecular Weight: 34 kDa

Gene ID: 5565

UniProt: O43741

Pathways: AMPK Signaling, Warburg Effect