POLR2A/RPB1 antibody (AA 1148-1384)

Catalog No. ABIN5693106

Product Details anti-POLR2A/RPB1 Antibody

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Binding Specificity: AA 1148-1384

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2A/RPB1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: Anti-RNA polymerase II RPB1/POLR2A Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RNA polymerase II RPB1/POLR2A Antibody Picoband® (ABIN5693106). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Immunogen: E. coli-derived human POLR2A recombinant protein (Position: A1148-H1384).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
ELISA, 0.1-0.5 μg/mL
1. "Entrez Gene: POLR2A polymerase (RNA) II (DNA ed) polypeptide A, 220 kDa". 2. Krum SA, Miranda GA, Lin C, Lane TF (December 2003). "BRCA1 associates with processive RNA polymerase II". J. Biol. Chem. 278 (52): 52012-20. 3. Scully R, Anderson SF, Chao DM, Wei W, Ye L, Young RA, Livingston DM, Parvin JD (May 1997). "BRCA1 is a component of the RNA polymerase II holoenzyme". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5605-10.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Alternative Name: POLR2A (POLR2A Products)

Background:

Synonyms: DNA-ed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-ed RNA polymerase II subunit A, DNA-ed RNA polymerase III largest subunit, RNA-ed RNA polymerase II subunit RPB1, POLR2A, POLR2

Background: DNA-ed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.

Molecular Weight: 120 kDa

Gene ID: 5430

UniProt: P24928

Pathways: Regulatory RNA Pathways