GALT antibody (AA 188-379)

Catalog No. ABIN5693112

This Rabbit Polyclonal antibody specifically detects GALT in WB, IHC, IP and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for GALT antibody (AA 188-379) (ABIN5693112)

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-GALT Antibody

Binding Specificity: AA 188-379

Purpose: Anti-GALT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GALT Antibody Picoband® (ABIN5693112). Tested in ELISA, Flow Cytometry, IP, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human GALT recombinant protein (Position: Q188-A379).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry, 2-5 μg/mL
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Kozak, L., Francova, H., Pijackova, A., Peskovova, K., Martincova, O., Krijt, J. Presence of a deletion in the 5-prime upstream region of the GALT gene in Duarte (D2) alleles. J. Med. Genet. 36: 576-578, 1999. 2. Tyfield, L., Reichardt, J., Fridovich-Keil, J., Croke, D. T., Elsas, L. J., II, Strobl, W., Kozak, L., Coskun, T., Novelli, G., Okano, Y., Zekanowski, C., Shin, Y., Boleda, M. D. Classical galactosemia and mutations at the galactose-1-phosphate uridyl transferase (GALT) gene.Hum. Mutat. 13: 417-430, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GALT

Target: GALT (Galactose-1-Phosphate Uridylyltransferase (GALT))

Alternative Name: GALT

Background:

Synonyms: Galactose-1-phosphate uridylyltransferase,Gal-1-P uridylyltransferase,2.7.7.12,UDP-glucose--hexose-1-phosphate uridylyltransferase,GALT,

Tissue Specificity: Widely expressed. Detected in adult colon epithelium.

Background: Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 48 kDa

Gene ID: 2592

UniProt: P07902