XRCC1 antibody (AA 538-633)

Catalog No. ABIN5693138

This anti-XRCC1 antibody is a Rabbit Polyclonal antibody detecting XRCC1 in WB, IHC, IF, ELISA, FACS, ICC and IHC (fro). Suitable for Human, Mouse and Rat.

Quick Overview for XRCC1 antibody (AA 538-633) (ABIN5693138)

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-XRCC1 Antibody

Binding Specificity: AA 538-633

Purpose: Anti-XRCC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-XRCC1 Antibody Picoband® (ABIN5693138). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human XRCC1 recombinant protein (Position: E538-A633).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Bhattacharyya, N., Banerjee, S. A novel role of XRCC1 in the functions of a DNA polymerase beta variant. Biochemistry 40: 9005-9013, 2001. 2. Brookman, K. W., Tebbs, R. S., Allen, S. A., Tucker, J. D., Swiger, R. R., Lamerdin, J. E., Carrano, A. V., Thompson, L. H. Isolation and characterization of mouse Xrcc1, a DNA repair gene affecting ligation. Genomics 22: 180-188, 1994.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for XRCC1

Target: XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1 (XRCC1))

Alternative Name: XRCC1

Background:

Synonyms: DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, XRCC1

Background: XRCC1 (X-RAY REPAIR, COMPLEMENTING DEFECTIVE, IN CHINESE HAMSTER, 1) is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The XRCC1 interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of XRCC1 on ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.

Molecular Weight: 90 kDa

Gene ID: 7515

UniProt: P18887

Pathways: DNA Damage Repair