Glutathione Reductase antibody (AA 256-522)

Catalog No. ABIN5693151

This Rabbit Polyclonal antibody specifically detects Glutathione Reductase in WB, ELISA, IF, ICC, IHC (p) and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Glutathione Reductase antibody (AA 256-522) (ABIN5693151)

Target: Glutathione Reductase (GSR)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glutathione Reductase antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-Glutathione Reductase Antibody

Binding Specificity: AA 256-522

Purpose: Anti-Glutathione Reductase/GSR Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-Glutathione Reductase/GSR Antibody Picoband® (ABIN5693151). Tested in ELISA, Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Glutathione Reductase recombinant protein (Position: K256-R522).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Deponte M (May 2013). "Glutathione catalysis and the reaction mechanisms of glutathione-dependent enzymes". Biochim. Biophys. Acta. 1830 (5): 3217-66. 2. Meister A (November 1988). "Glutathione metabolism and its selective modification". J. Biol. Chem. 263 (33): 17205-8.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Glutathione Reductase

Target: Glutathione Reductase (GSR)

Alternative Name: GSR

Background:

Synonyms: Glutathione reductase, mitochondrial, GR, GRase, GSR, GLUR, GRD1

Tissue Specificity: Expressed in peripheral blood leukocytes.

Background: Glutathione reductase (GR), also known as glutathione-disulfide reductase (GSR), is an enzyme that in humans is encoded by the GSR gene. This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Molecular Weight: 52 kDa

Gene ID: 2936

UniProt: P00390

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis