CCT3 antibody (C-Term)

Catalog No. ABIN5693184

This Mouse Monoclonal antibody specifically detects CCT3 in WB, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for CCT3 antibody (C-Term) (ABIN5693184)

Target: CCT3 (Chaperonin Containing TCP1, Subunit 3 (Gamma) (CCT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCT3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 12H4

Product Details anti-CCT3 Antibody

Binding Specificity: C-Term

Purpose: Anti-CCT3 Antibody (monoclonal, 12H4)

Sequence: EPLAVKLQTY KTAVETAVLL LRIDDIVSGH KKKGDDQSRQ

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse,rat sequences by one amino acid.

Characteristics: Anti-CCT3 Antibody.(monoclonal, 12H4). Tested in IF, ICC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CCT3, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunocytochemistry/Immunofluorescence, 5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CCT3

Target: CCT3 (Chaperonin Containing TCP1, Subunit 3 (Gamma) (CCT3))

Alternative Name: CCT3

Background:

Background: T-complex protein 1 subunit gamma is a protein that in humans is encoded by the CCT3 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8.

Gene Full Name: chaperonin containing TCP1 subunit 3

Molecular Weight: 60 kDa

Gene ID: 7203

UniProt: P49368