ITGA5 antibody (AA 42-263)

Catalog No. ABIN5693202

This Rabbit Polyclonal antibody specifically detects ITGA5 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 4+ publications.

Quick Overview for ITGA5 antibody (AA 42-263) (ABIN5693202)

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ITGA5 Antibody

Binding Specificity: AA 42-263

Purpose: Anti-Integrin alpha 5/ITGA5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Integrin alpha 5/ITGA5 Antibody Picoband® (ABIN5693202). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human Integrin alpha 5 recombinant protein (Position: F42-A263).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Sosnoski DM, Emanuel BS, Hawkins AL, van Tuinen P, Ledbetter DH, Nussbaum RL, Kaos FT, Schwartz E, Phillips D, Bennett JS, et al. (Aug 1988). 2. "Entrez Gene: ITGA5 integrin, alpha 5 (fibronectin receptor, alpha polypeptide)".

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-ITGA5 antibody (ABIN5693202)

Chen, Sun, Liu, Tong, Meng: "Silencing of Paternally Expressed Gene 10 Inhibits Trophoblast Proliferation and Invasion." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144845, (2015) (PubMed).

Zhang, Ren, Luo, Fan, Liu, Liang, Ma, Yu, Bai: "A small physiological electric field mediated responses of extravillous trophoblasts derived from HTR8/SVneo cells: involvement of activation of focal adhesion kinase signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92252, (2014) (PubMed).

Huang, Lian, Chen, Yang, Yang: "Characterization of rat hair follicle stem cells selected by vario magnetic activated cell sorting system." in: Acta histochemica et cytochemica, Vol. 42, Issue 5, pp. 129-36, (2009) (PubMed).

"College training and examinations." in: The Australian and New Zealand journal of psychiatry, Vol. 23, Issue 2, pp. 148-54, (1989) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: ITGA5

Background:

Synonyms: Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA

Tissue Specificity: Highest expression in the prostate, small intestine, colon, kidney and liver.

Background: Integrin alpha-5, also known as FNRA or VLA5A, is a protein that in humans is encoded by the ITGA5 gene. The product of this gene belongs to the integrin alpha chain family. Integrins are integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.

Molecular Weight: 150 kDa

Gene ID: 3678

UniProt: P08648

Pathways: Integrin Complex