CDC45 antibody (AA 166-431)

Catalog No. ABIN5693209

Product Details anti-CDC45 Antibody

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Binding Specificity: AA 166-431

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC45 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Brand: Picoband™

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CDC45L detection. Tested with WB, Direct ELISA in Human,Mouse,Rat.

Immunogen: E. coli-derived human CDC45L recombinant protein (Position: E166-A431).

Application Details

Application Notes:

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Application Details: Western blot, 0.1-0.5 μg/mL
Direct ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Target Details for CDC45

Target: CDC45 (Cell Division Cycle 45 Homolog (S. Cerevisiae) (CDC45))

Alternative Name: CDC45 (CDC45 Products)

Synonyms: cdc45l antibody, cdc45l2 antibody, porc-pi-1 antibody, CDC45L antibody, CDC45L2 antibody, PORC-PI-1 antibody, Cdc45l antibody, cell division cycle 45 antibody, cell division cycle 45 S homeolog antibody, cell division cycle 45 L homeolog antibody, cdc45 antibody, CDC45 antibody, cdc45.S antibody, Cdc45 antibody, cdc45.L antibody

Background:

Synonyms: Cell division control protein 45 homolog, PORC-PI-1, CDC45, CDC45L, CDC45L2, UNQ374/PRO710

Tissue Specificity: Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.

Background: CDC45 is a protein that in humans is encoded by the CDC45L gene. The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

UniProt: O75419

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA