MDC1 antibody (AA 534-626)

Catalog No. ABIN5693232

This Rabbit Polyclonal antibody specifically detects MDC1 in IHC, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for MDC1 antibody (AA 534-626) (ABIN5693232)

Target: MDC1 (Mdc1 (MDC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDC1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MDC1 Antibody

Binding Specificity: AA 534-626

Purpose: Anti-MDC1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MDC1 Antibody Picoband® (ABIN5693232). Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

Immunogen: E. coli-derived human MDC1 recombinant protein (Position: A534-Q626).

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Dimitrova, N., de Lange, T. MDC1 accelerates nonhomologous end-joining of dysfunctional telomeres. Genes Dev. 20: 3238-3243, 2006. 2. Goldberg, M., Stucki, M., Falck, J., D'Amours, D., Rahman, D., Pappin, D., Bartek, J., Jackson, S. P. MDC1 is required for the intra-S-phase DNA damage checkpoint. Nature 421: 952-956, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for MDC1

Target: MDC1 (Mdc1 (MDC1))

Alternative Name: MDC1

Background:

Synonyms: Mediator of DNA damage checkpoint protein 1, Nuclear factor with BRCT domains 1, MDC1, KIAA0170, NFBD1

Tissue Specificity: Highly expressed in testis.

Background: Mediator of DNA damage checkpoint protein 1 is a 2080 amino acid long protein that in humans is encoded by the MDC1 gene. The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.

Molecular Weight: 50 kDa

Gene ID: 9656

UniProt: Q14676