Prothrombin antibody (AA 67-124)

Catalog No. ABIN5693261

Cited in 1 publication. The Rabbit Polyclonal anti-Prothrombin antibody (ABIN5693261) specifically detects Prothrombin in WB, IHC, ICC, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for Prothrombin antibody (AA 67-124) (ABIN5693261)

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prothrombin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISA, Flow Cytometry (FACS)

Product Details anti-Prothrombin Antibody

Binding Specificity: AA 67-124

Purpose: Anti-Prothrombin/F2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Prothrombin detection. Tested with WB, IHC-P, Direct ELISA in Human.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Prothrombin recombinant protein (Position: Y67-R124).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-Prothrombin antibody (ABIN5693261)

Shen, Fang, Lv, Pei, Wang, Jiang, Ding: "Heparin impairs angiogenesis through inhibition of microRNA-10b." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26616-27, (2011) (PubMed).

Target Details for Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Alternative Name: F2

Background:

Synonyms: Prothrombin, Coagulation factor II, Activation peptide fragment 1, Activation peptide fragment 2, Thrombin light chain, Thrombin heavy chain, F2

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Molecular Weight: 85 kDa

UniProt: P00734

Pathways: Complement System, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling