DPYD antibody (AA 356-511)

Catalog No. ABIN5693320

The Rabbit Polyclonal anti-DPYD antibody has been validated for WB and ELISA. It is suitable to detect DPYD in samples from Human, Mouse and Rat.

Quick Overview for DPYD antibody (AA 356-511) (ABIN5693320)

Target: DPYD (Dihydropyrimidine Dehydrogenase (DPYD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPYD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DPYD Antibody

Binding Specificity: AA 356-511

Purpose: Anti-DPYD Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-DPYD Antibody Picoband® (ABIN5693320). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: E. coli-derived human DPYD recombinant protein (Position: A356-Y511).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Albin, N., Johnson, M. R., Shahinian, H., Wang, K., Diasio, R. B. Initial characterization of the molecular defect in human dihydropyrimidine dehydrogenase deficiency. Proc. Am. Assoc. Cancer Res. (Abstract) 36: 211 only, 1995. 2. McLeod, H. L., Collie-Duguid, E. S. R., Vreken, P., Johnson, M. R., Wei, X., Sapone, A., Diasio, R. B., Fernandez-Salguero, P., van Kuilenberg, A. B. P., van Gennip, A. H., Gonzales, F. J. Nomenclature for human DPYD alleles. Pharmacogenetics 8: 455-459, 1998. 3. Ridge, S. A., Brown, O., McMurrough, J., Fernandez-Salguero, P., Evans, W. E., Gonzalez, F. J., McLeod, H. L. Mutations at codon 974 of theDPYD gene are a rare event. Brit. J. Cancer 75: 178-179, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for DPYD

Target: DPYD (Dihydropyrimidine Dehydrogenase (DPYD))

Alternative Name: DPYD

Background:

Synonyms: Dihydropyrimidine dehydrogenase [NADP (+)], DHPDHase, DPD, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPYD

Tissue Specificity: Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells.

Background: DPYD (Dihydropyrimidine Dehydrogenase), also called DPD, is an enzyme that in humans is encoded by the DPYD gene. The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. The structure of the DPYD gene contains 23 exons spanning about 950 kb. Using somatic cell hybrid strategies, the DPYD gene is mapped to the centromeric region of chromosome 1 between 1p22 and 1q21. By fluorescence in situ hybridization, the DPYD gene is mapped to 1p22. The highest level of DPD was found in monocytes followed by that in lymphocytes, granulocytes, and platelets, whereas no significant activity of DPD could be detected in erythrocytes. The activity of DPD in peripheral blood mononuclear cells was intermediate between that observed in monocytes and lymphocytes. By cDNA microarray, Western blot analysis, and luciferase reporter assay, the transcription factor LSF was identified as a positive regulator of DPYD.

Molecular Weight: 111 kDa

Gene ID: 1806

UniProt: Q12882

Pathways: Ribonucleoside Biosynthetic Process