LDHB antibody

Catalog No. ABIN5698058

The Rabbit Polyclonal anti-LDHB antibody is suitable to detect LDHB in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IP, IF, ELISA and FACS.

Quick Overview for LDHB antibody (ABIN5698058)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Product Details anti-LDHB Antibody

Purpose: LDHB antibody

Immunogen: lactate dehydrogenase B

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

mouse brain tissue were subjected to SDS PAGE followed by western blot with FNab04738(LDHB antibody) at dilution of 1:2000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Alternative Name: LDHB

Background:

Synonyms: L-lactate dehydrogenase B chain (LDH-B)|LDH heart subunit (LDH-H)|Renal carcinoma antigen NY-REN-46|LDHB

Background: This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

Molecular Weight: 35 kDa

Gene ID: 3945

UniProt: P07195

Pathways: Warburg Effect