MMP2 antibody

Catalog No. ABIN5699311

This Rabbit Polyclonal antibody specifically detects MMP2 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human, Rat, Mouse and Zebrafish (Danio rerio).

Quick Overview for MMP2 antibody (ABIN5699311)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-MMP2 Antibody

Immunogen: matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

Isotype: IgG

Application Details

Application Notes: WB : 1:200-1:2000 IP : 1:200-1:1000 IHC : 1:100-1:400 IF : 1:50-1:500

Comment:

MCF7 cells were subjected to SDS PAGE followed by western blot with FNab05238(MMP2 antibody) at dilution of 1:500

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Expiry Date: 12 months

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2

Background: Synonyms:72 kDa gelatinase, 72 kDa type IV collagenase, CLG4, CLG4A, Gelatinase A, Matrix metalloproteinase 2, MMP 2, MMP II, MMP2, MONA, TBE 1 Background:This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 72 kDa, 60 kDa

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response