MAP1LC3A antibody (AA 1-125)

Catalog No. ABIN5700262

This Rabbit Polyclonal antibody specifically detects MAP1LC3A in WB, ELISA, IHC, IF, IP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MAP1LC3A antibody (AA 1-125) (ABIN5700262)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-MAP1LC3A Antibody

Binding Specificity: AA 1-125

Purpose: LC3 antibody

Immunogen:

Immunogen sequence: 1-125aa

Immunogen: microtubule-associated protein 1 light chain 3 beta

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab04717(LC3 Antibody) at dilution of 1:1000

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.1 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3

Background:

Synonyms: Microtubule-associated proteins 1A/1B light chain 3B|Autophagy-related protein LC3 B|Autophagy-related ubiquitin-like modifier LC3 B|MAP1 light chain 3-like protein 2|MAP1A/MAP1B light chain 3 B (MAP1A/MAP1B LC3 B)|Microtubule-associated protein 1 light chain 3 beta|MAP1LC3B|MAP1ALC3

Background: Map1LC3, also known as LC3, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18- kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 16- kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.

Molecular Weight: 16 kDa

Pathways: Autophagy