ALKBH5 antibody

Catalog No. ABIN5705091

The Rabbit Polyclonal anti-ALKBH5 antibody (ABIN5705091) specifically detects ALKBH5 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ALKBH5 antibody (ABIN5705091)

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ALKBH5 Antibody

Purpose: ALKBH5 antibody

Immunogen: alkB, alkylation repair homolog 5 (E. coli)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IHC: 1:20-1:200

Comment:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab00314(ALKBH5 antibody) at dilution of 1:500

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ALKBH5

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Alternative Name: ALKBH5

Background:

Synonyms: RNA demethylase ALKBH5|Alkylated DNA repair protein alkB homolog 5|Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5|ALKBH5|ABH5|OFOXD1

Background: Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine(m6A) RNA, the most prevalent internal modification of messenger RNA(mRNA) in higher eukaryotes(PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Can also demethylate N(6)-methyladenosine in single-stranded DNA(in vitro)(PubMed:24616105). Requires molecular oxygen, alpha-ketoglutarate and iron(PubMed:21264265, PubMed:23177736, PubMed:24778178, PubMed:24616105, PubMed:24489119). Demethylation of m6A mRNA affects mRNA processing and export(PubMed:23177736). Required for spermatogenesis(By similarity).

Molecular Weight: 51kDa

Gene ID: 54890

UniProt: Q6P6C2