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Biotin antibody

WB, FACS, ELISA, IHC, FM Host: Mouse Monoclonal 4C7-D5 unconjugated

Catalog No. ABIN5706828

Datasheet as PDF

Target See all Biotin products

Biotin

Reactivity

Please inquire
Host
54

33

27
Mouse
Compatible Secondaries
Clonality
72

35
Monoclonal
Conjugate
28

9

8

7

7

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Biotin antibody is un-conjugated
Application
54

45

43

33

30

18

14

13

12

12

9

9

6

4

4

3

2

2

2

2

2

1

1

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Characteristics

Synonyms: mouse anti-Biotin Monoclonal Antibody, Biotin Antibody, Vitamin H, Coenzyme R
Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.

Purification

Anti-Biotin Antibody was prepared from mouse ascites by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-mouse serum, Biotin conjugated IgG and Biotin conjugated Bovine Serum Albumin.

Immunogen

Immunogen: Anti-Biotin Antibody was produced by repeated immunizations with Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH).
Immunogen Type: Other

Clone

4C7-D5

Isotype

IgG2a
anti-Biotin antibody
WB, ELISA, IP, DB Host: Rabbit Polyclonal unconjugated

anti-Biotin antibody
ELISA Host: Goat Polyclonal unconjugated Fab fragment

anti-Biotin antibody
WB, FACS, IHC, IF, ISt, CM Host: Mouse Monoclonal Hyb-8 unconjugated

anti-Biotin antibody
IHC, ISt Host: Rabbit Monoclonal BTN-2032R unconjugated Recombinant Antibody

anti-Biotin antibody (DyLight 488)
WB, FM, FLISA Host: Goat Polyclonal DyLight 488

anti-Biotin antibody
FACS, ELISA Host: Mouse Monoclonal SB58c unconjugated

anti-Biotin antibody
WB, FACS, ELISA, IHC, IP, Lbl Host: Goat unconjugated

anti-Biotin antibody
IP, Con Host: Mouse Monoclonal unconjugated

anti-Biotin antibody
FACS, ELISA, IP, CyTOF Host: Mouse Monoclonal 1D4-C5 unconjugated

anti-Biotin antibody
IP, Con Host: Rabbit Polyclonal unconjugated

Application Notes

Flow Cytometry Dilution: 1-2 μg/mL
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Biotin antibody is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Western Blot Dilution: 1:2,000 - 1:10,000
ELISA Dilution: 1:20,000
IF Microscopy Dilution: 2 μg/mL

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2
Stabilizer: None

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

12 months
Target

Biotin

Abstract

Biotin Products

Target Type

Chemical