PKM antibody

Catalog No. ABIN5706831

Supplier Product No.: 600-401-gv5

This anti-PKM antibody is a Rabbit Polyclonal antibody detecting PKM in ELISA and WB. Suitable for Human.

Quick Overview for PKM antibody (ABIN5706831)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKM antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-PKM Antibody

Supplier Product No.: 600-401-gv5

Supplier: Rockland

Purpose: PKM2 Antibody

Cross-Reactivity (Details): Anti-PKM2 is directed against human PKM2 at an internal position.

Characteristics: Synonyms: rabbit anti-PKM2 antibody, PKM 2, PKM-2, Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, OIP3, OIP-3, PK2, PK3

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Sterility: Sterile filtered

Immunogen:

Immunogen: Affinity purified Anti-PKM2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the internal portion of human PKM2 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band 56-58 kDa in size corresponding to PKM2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 5 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM

Background: Background: Anti-PKM2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). Pyruvate kinase has been shown to be expressed in tumor, embryonic and normal proliferating cells in varying levels. Studies show that when PKM2 is secreted it can induce EGFR phosphorylation which stimulates the EGFR downstream pathway. Previously PKM has been shown to be critical for aerobic glycolysis. Four isoforms of PKM play alternative roles within the system and post-translational modifications along these isoforms induce multiple pathways making this Anti-PKM2 antibody ideal for investigators involved in cancer research and post-translational modifications.

Gene ID: 5315

NCBI Accession: NP_001193725

UniProt: P14618

Pathways: Warburg Effect