SPS antibody

Catalog No. ABIN5706929

There are 2+ publications for this product available. The Rabbit Polyclonal anti-SPS antibody is suitable to detect SPS in samples from Arabidopsis thaliana, Barley, Colobanthus quitensis, Pinus strobus, Potato, Tomato, Triticum aestivum, Zea mays, Miscanthus giganteus and Rape. It has been validated for WB.

Quick Overview for SPS antibody (ABIN5706929)

Target: SPS

Reactivity: Arabidopsis thaliana, Barley, Colobanthus quitensis, Pinus strobus, Potato, Tomato, Triticum aestivum, Zea mays, Miscanthus giganteus, Rape

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Purpose: Anti-SPS | Sucrose phosphate synthase, global

Cross-Reactivity (Details): Arabidopsis thaliana, Brassica napus, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Miscanthus x giganteus, Pinus strobus, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Citrus sinensis,Glycine max,Nicotiana benthamiana,Nicotiana tabacum,Oryza sativa,Physcomitrium patens,Populus balsamifera,Robinia pseudoacaci,Ricinus communis,Saccharum officinarum,Solanum lycopersicum,Theobroma cacao,Vicia faba,Vitis vinifera

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences

Application Details

Application Notes: 1 : 1000-1 : 5000 (WB)

Comment:

Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 25 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Yancey et al.: "Commentary: Omalizumab therapy for bullous pemphigoid by KK Yu, AB Crew, KAN Messingham, JA Fairley, DT Woodley. J Am Acad Dermatol 2014 Jun 20. pii: S0190-9622(14)01410-8. doi: ..." in: Dermatologic therapy, Vol. 28, Issue 1, pp. 3, (2015) (PubMed).

Whittaker, Martinelli, Farrant, Bochicchio, Vazzana: "Sucrose phosphate synthase activity and the co-ordination of carbon partitioning during sucrose and amino acid accumulation in desiccation-tolerant leaf material of the C4 resurrection plant Sporobolus stapfianus during dehydration." in: Journal of experimental botany, Vol. 58, Issue 13, pp. 3775-87, (2007) (PubMed).

Target Details

Target: SPS

Background: SPS (sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.

Molecular Weight: 120 | 120-130 kDa (fragments of 30-90 kDa may be detected)

Gene ID: 832150

UniProt: Q94BT0, Q67WN8, Q43845