SHMT1 antibody

Catalog No. ABIN5706931

The Rabbit Polyclonal anti-SHMT1 antibody has been validated for WB. It is suitable to detect SHMT1 in samples from Vicia faba, Spinach, Pisum sativum and Arabidopsis thaliana. There is 1 publication available.

Quick Overview for SHMT1 antibody (ABIN5706931)

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Reactivity: Vicia faba, Spinach, Pisum sativum, Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHMT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SHMT1 Antibody

Specificity: The antibody is very specific for salmonid inducible form - hsp70.

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: dicots including: Glycine max, Medicago truncatula, Ricinus communis, Solanum tuberosum, Vitis vinifera, monocots: Hordeum vulgare, trees: Populus balsamifera, algae: Chlamydomonas reinhardii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 53 kDa (Arabidopsis thaliana)

Purification: affinity purified

Immunogen: Purified SHMT protein from Spinacia oleracea

Isotype: IgG

Application Details

Application Notes: 1:5 000 with standard ECL (WB)

Comment:

this antibody can be used on total leaf extracts and isolated mitochondria

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-SHMT1 antibody (ABIN5706931)

Wei, Sun, Sandoval, Cross, Gordon, Kang, Roje: "Folate polyglutamylation eliminates dependence of activity on enzyme concentration in mitochondrial serine hydroxymethyltransferases from Arabidopsis thaliana." in: Archives of biochemistry and biophysics, Vol. 536, Issue 1, pp. 87-96, (2013) (PubMed).

Target Details for SHMT1

Target: SHMT1 (serine Hydroxymethyltransferase 1 (Soluble) (SHMT1))

Alternative Name: SHMT

Background: Serine hydroxymethyltransferase (SHMT) is part of the mitochondrial enzyme complex.

Molecular Weight: 53 kDa (Arabidopsis thaliana)