AadA1 antibody

Catalog No. ABIN5706932

There is 1 publication for this product available. The Rabbit Polyclonal anti-AadA1 antibody is suitable to detect AadA1 in samples from Chlamydomonas reinhardtii and Nicotiana tabacum. It has been validated for WB.

Quick Overview for AadA1 antibody (ABIN5706932)

Target: AadA1 (aadA1) (Aminoglycoside adenyltransferase (aadA1))

Reactivity: Chlamydomonas reinhardtii, Nicotiana tabacum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AadA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-AadA1 Antibody

Purpose: Anti-aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

Specificity: AadA cassette in Nicotiana tabacum.

Cross-Reactivity (Details): aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Acinetobacter baumannii,Enterobacter agglomerans,Klebsiella pneumoniae,Klebsiella oxytoca,Kluyvera ascorbata,Pseudomonas aeruginosa,Salmonella enterica subsp. enterica serovar Worthington,Staphylococcus aureus,Serratia sp.,Shigella flexneri

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated peptide derived from known aadA1 protein sequences

Application Details

Application Notes: 1 : 3 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-AadA1 antibody (ABIN5706932)

Kolotilin, Kaldis, Pereira, Laberge, Menassa: "Optimization of transplastomic production of hemicellulases in tobacco: effects of expression cassette configuration and tobacco cultivar used as production platform on recombinant protein yields." in: Biotechnology for biofuels, Vol. 6, Issue 1, pp. 65, (2013) (PubMed).

Target Details for AadA1

Target: AadA1 (aadA1) (Aminoglycoside adenyltransferase (aadA1))

Background:

Background: aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.

Additional information: This product can be sold containing ProClin if requested

Molecular Weight: 30 | 30 kDa