HMGCR antibody (Internal Region)

Catalog No. ABIN570699

The Goat Polyclonal anti-HMGCR antibody (ABIN570699) specifically detects HMGCR in ELISA, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for HMGCR antibody (Internal Region) (ABIN570699)

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HMGCR antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-HMGCR Antibody

Binding Specificity: Internal Region

Purpose: HMGCR / LDLCQ3

Sequence: ADPSPQNSTA DTSK

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ADPSPQNSTADTSK , from the internal region of the protein sequence according to NP_000850.1.

Isotype: IgG

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and vesicles of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Alternative Name: HMGCR

Background: HMGCR, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase, LDLCQ3, 3-hydroxy-3-methylglutaryl CoA reductase (NADPH), hydroxymethylglutaryl-CoA reductase, HMG-CoA reductase

Gene ID: 3156

NCBI Accession: NP_000850

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha