E2F8 antibody (Internal Region)

Catalog No. ABIN570707

The Goat Polyclonal anti-E2F8 antibody has been validated for ELISA, FACS and IF. It is suitable to detect E2F8 in samples from Human.

Quick Overview for E2F8 antibody (Internal Region) (ABIN570707)

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This E2F8 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-E2F8 Antibody

Binding Specificity: Internal Region

Purpose: E2F8

Sequence: KSIESDRRKI NSAPS

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KSIESDRRKINSAPS , from the internal region of the protein sequence according to NP_078956.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 80 kDa in Human K562 and Mouse Neuro2a cell lysate after 0.3 μg/mL antibody staining. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended conc

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for E2F8

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Alternative Name: E2F8

Background: E2F8, E2F transcription factor 8, FLJ23311, E2F family member 8

Gene ID: 79733, 108961

NCBI Accession: NP_078956