E2F8 antibody (AA 835-865)

Catalog No. ABIN6242297

This Rabbit Polyclonal antibody specifically detects E2F8 in WB. It exhibits reactivity toward Human.

Quick Overview for E2F8 antibody (AA 835-865) (ABIN6242297)

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F8 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: RB54144

Product Details anti-E2F8 Antibody

Binding Specificity: AA 835-865

Predicted Reactivity: M, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This E2F8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 835-865 amino acids from human E2F8.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for E2F8

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Alternative Name: E2F8

Background: Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.

Molecular Weight: 94166

UniProt: A0AVK6