FOXP3 antibody (N-Term) (FITC)

Catalog No. ABIN5707213

The FITC-conjugated Rat Monoclonal anti-FOXP3 antibody (Clone SB168a) (ABIN5707213) specifically detects FOXP3 in FACS and IHC (p). The antibody is reactive with Mouse samples.

Quick Overview for FOXP3 antibody (N-Term) (FITC) (ABIN5707213)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SB168a

Product Details anti-FOXP3 Antibody (FITC)

Binding Specificity: N-Term

Purpose: Rat Anti-Mouse Foxp3-FITC

Specificity: Mouse Foxp3 (Mr ~57 kDa). Does not cross react with Human Foxp3. T regulatory cells (Originally called T suppressor cells) are a subpopulation of T cells. Their major roles are to suppress excess immune response of other cells and to maintain tolerance of self-antigens. Initially, researchers used CD4 and CD25 (IL-2 receptor) to identify T suppress subpopulation and purified CD4+CD25+ T cells were used for T cells Suppression Assay, however some other activated T cells also express CD25.1,2 The most convincible molecular marker of Treg cells is Forkhead box protein 3 (Foxp3) which is expressed in the nucleus.3 Anti-Foxp3 antibodies are now widely applied to identify the roles of Treg cells in the development of many diseases, such as cancer, autoimmune disease, and organ transplantation.4,5,6 Scientists found that the over-activation of Treg cells may cause cancer cells to escape from immune surveillance 5. Conversely, patients with autoimmune diseases such as psoriasis, systemic lupus erythematous (SLE) have relative dysfunction of Foxp3 positive cells.7,8 Immunologists are trying to induce immune tolerance in organ transplantation using Treg cells.6

Characteristics: FITC Anti-Mouse Foxp3 antibody for use in flow cytometry and immunohistochemistry assays.

Immunogen: N-terminal recombinant mouse Foxp3

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline containing < 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Foxp3

Background:

Description: Rat Anti-Mouse Foxp3-FITC

NCBI_Official_Symbol: Foxp3

Synoynms: Forkhead box protein 3

Gene ID: 20371

UniProt: Q99JB6

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation