ATG4D antibody (Internal Region)

Catalog No. ABIN570736

This Goat Polyclonal antibody specifically detects ATG4D in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for ATG4D antibody (Internal Region) (ABIN570736)

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATG4D antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-ATG4D Antibody

Binding Specificity: Internal Region

Purpose: ATG4D

Sequence: CSQLAQPTLR LPRT

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CSQLAQPTLRLPRT, from the internal region (near C terminus) of the protein sequence according to NP_116274.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Pancreas. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx. 26 kDa band in Human Skeletal Muscle lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATG4D

Target: ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))

Alternative Name: ATG4D

Background: ATG4D, ATG4 autophagy related 4 homolog D (S. cerevisiae), APG4-D, APG4D, AUTL4, APG4 autophagy 4 homolog D, AUT-like 4, cysteine endopeptidase, autophagin-4, autophagy-related cysteine endopeptidase 4, cysteine protease involved in autophagy

Gene ID: 84971, 235040, 686505

NCBI Accession: NP_116274

Pathways: Autophagy