MTHFR antibody (C-Term)

Catalog No. ABIN570752

The Goat Polyclonal anti-MTHFR antibody has been validated for ELISA and IHC. It is suitable to detect MTHFR in samples from Human.

Quick Overview for MTHFR antibody (C-Term) (ABIN570752)

Target: MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MTHFR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MTHFR Antibody

Binding Specificity: C-Term

Purpose: MTHFR

Sequence: NRPTQNARET EAP

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NRPTQNARETEAP, from the C Terminus of the protein sequence according to NP_005948.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 28 kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MTHFR

Target: MTHFR (Methylenetetrahydrofolate Reductase (NAD(P)H) (MTHFR))

Alternative Name: MTHFR

Background: MTHFR, 5,10-methylenetetrahydrofolate reductase (NADPH), OTTHUMP00000002367, OTTHUMP00000002368, methylenetetrahydrofolate reductase intermediate form

Gene ID: 4524

NCBI Accession: NP_005948

Pathways: Methionine Biosynthetic Process