CLIP antibody (AA 25-39)

Catalog No. ABIN5707527

The Rabbit Monoclonal anti-CLIP antibody has been validated for IHC (p). It is suitable to detect CLIP in samples from Human, Rat and Mouse.

Quick Overview for CLIP antibody (AA 25-39) (ABIN5707527)

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CLIP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CLIP-2040R

Product Details anti-CLIP Antibody

Binding Specificity: AA 25-39

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone (CLIP sequence) were used as the immunogen for this ACTH antibody.

Isotype: IgG kappa

Application Details

Application Notes: The optimal dilution of the recombinant ACTH antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format : incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant ACTH antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for CLIP

Target: CLIP (Corticotropin-Like Intermediate Peptide (CLIP))

Alternative Name: ACTH / CLIP

Background: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 17-39 of ACTH) and does not react with Synacthen (aa 1-24 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.