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CLIP antibody (AA 25-39)

CLIP Reactivity: Human FACS, IF, IHC (p), ELISA Host: Mouse Monoclonal ADCT1-1 unconjugated
Catalog No. ABIN3024113
  • Target See all CLIP products
    CLIP (Corticotropin-Like Intermediate Peptide (CLIP))
    Binding Specificity
    • 3
    • 2
    • 2
    AA 25-39
    Reactivity
    • 9
    • 4
    • 2
    Human
    Host
    • 6
    • 5
    Mouse
    Clonality
    • 8
    • 3
    Monoclonal
    Conjugate
    • 9
    • 1
    • 1
    This CLIP antibody is un-conjugated
    Application
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
    Purification
    Protein G affinity chromatography
    Immunogen
    Amino acids 25-39 (NGAEDESAEAFPLEF) of Adrenocorticotropic hormone were used as the immunogen for this ACTH antibody.
    Clone
    ADCT1-1
    Isotype
    IgG1
  • Application Notes
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ACTH antibody to be titered up or down for optimal performance.

    1. Staining of FFPE tissue requires boiling sections in 10  mM Citrate Buffer,  pH 6, for 10-20 min followed by cooling at RT for 20 min.\. ELISA: order BSA free format for coating,FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE):0.5-1 μg/mL

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the ACTH antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    CLIP (Corticotropin-Like Intermediate Peptide (CLIP))
    Alternative Name
    ACTH / CLIP (CLIP Products)
    Background
    ACTH (or Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This mAb is specific to CLIP (aa 25-39) and does not react with Synacthen (aa 1-24). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
    Gene ID
    5443
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