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EpCAM antibody (Extracellular Domain)

This anti-EpCAM antibody is a Rabbit Monoclonal antibody detecting EpCAM in IHC (p). Suitable for Human.
Catalog No. ABIN5707558

Quick Overview for EpCAM antibody (Extracellular Domain) (ABIN5707558)

Target

See all EpCAM (EPCAM) Antibodies
EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity

  • 373
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Human

Host

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Rabbit

Clonality

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Monoclonal

Conjugate

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This EpCAM antibody is un-conjugated

Application

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Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

EGP40-2041R
  • Binding Specificity

    • 91
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    • 32
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    AA 100-224, Extracellular Domain

    Purification

    Purified

    Purity

    Protein A affinity chromatography

    Immunogen

    A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.

    Isotype

    IgG kappa
  • Application Notes

    Titering of the recombinant EpCAM antibody may be required for optimal performance.

    1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

    Restrictions

    For Research Use only
  • Buffer

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    Preservative

    Azide free

    Storage

    4 °C,-20 °C

    Storage Comment

    Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
  • Target

    EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

    Alternative Name

    EpCAM

    Background

    EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas, in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]
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