SKA3 antibody (Internal Region)

Catalog No. ABIN570773

The Goat Polyclonal anti-SKA3 antibody has been validated for ELISA and IHC. It is suitable to detect SKA3 in samples from Human.

Quick Overview for SKA3 antibody (Internal Region) (ABIN570773)

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SKA3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SKA3 Antibody

Binding Specificity: Internal Region

Purpose: SKA3

Sequence: PLSKTNSSSN DLE

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PLSKTNSSSNDLE, from the internal region of the protein sequence according to NP_659498.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary experiments gave an approx 35 kDa and 150 kDa band in HeLa lysates after 2 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 46

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SKA3

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Alternative Name: C13orf3

Background: C13orf3, chromosome 13 open reading frame 3, MGC4832, RAMA1, SKA3

Gene ID: 221150, 219114, 361047

NCBI Accession: NP_659498